human adipose tissue derived mscs  (PromoCell)

Journal: Regenerative Therapy

Article Title: Mesenchymal stromal cell administration promotes macrophage-mediated bile duct regeneration

doi: 10.1016/j.reth.2026.101065

Figure Lengend Snippet: MSC treatment increases scar-healing macrophages during bile duct regeneration (A) Experimental outline for MSC treatment. (B–D) Histological and immunofluorescence images show broader CK19 + ductular expansion in mice receiving MSCs compared to controls. (E–G) MSC administration is associated with increased populations of Sox9 + and Ki67 + regenerative epithelial cells, indicating an enhanced biliary repair response.

Article Snippet: Human adipose tissue-derived MSCs (passage 2) were obtained from PromoCell (cat#: C-12977, Heidelberg, Germany) and expanded until passage 4 using StemPro MSC SFM XenoFree medium (Thermo Fisher Scientific, Waltham, MA, USA) at 37 °C.

Techniques: Immunofluorescence

Journal: Regenerative Therapy

Article Title: Mesenchymal stromal cell administration promotes macrophage-mediated bile duct regeneration

doi: 10.1016/j.reth.2026.101065

Figure Lengend Snippet: Macrophage depletion negates MSC treatment-induced improvements in bile duct regeneration (A) Schematic illustration of the experimental design, including MSC administration and clodronate liposome-mediated macrophage depletion. (B–D) Clodronate treatment in combination with MSCs resulted in marked body weight loss compared with MSC treatment alone. (E) Immunofluorescence imaging demonstrated that the depletion of macrophages attenuated MSC-associated bile duct regeneration, as shown by a reduction in CK19 + ductular structures and decreased Ki67 + proliferating cells within CK19 - regions. (F) Sox9 + cells within the CK19 - compartment were also diminished in the MSC + Clip group. (G) Active YAP + cells within CK19 + biliary areas were reduced following macrophage depletion, indicating that macrophages contribute to the MSC-associated enhancement of biliary regenerative signaling.

Article Snippet: Human adipose tissue-derived MSCs (passage 2) were obtained from PromoCell (cat#: C-12977, Heidelberg, Germany) and expanded until passage 4 using StemPro MSC SFM XenoFree medium (Thermo Fisher Scientific, Waltham, MA, USA) at 37 °C.

Techniques: Immunofluorescence, Imaging

Journal: Bioengineering

Article Title: Mesenchymal Stem Cell-Mediated Targeted Drug Delivery Systems for Hepatocellular Carcinoma: Current Advances and Future Directions

doi: 10.3390/bioengineering12111206

Figure Lengend Snippet: Mechanism of natural homing of mesenchymal stem cells to the tumor microenvironment. This illustration captures the natural homing journey of mesenchymal stem cells (MSCs, in blue) to the hepatocellular carcinoma microenvironment. Originating in the surrounding tissue (top), MSCs express receptors like CXCR4 and CCR2. This directed migration is propelled by chemoattractant gradients (SDF-1, MCP-1, and CXCL12), guiding the cells to the tumor site (bottom, light blue).

Article Snippet: Adipose tissue-derived MSCs (AT-MSCs) , Exosome-mediated miR-122 delivery , Sorafenib , Exosomes regulated target gene expression via miR-122, enhancing hepatocellular carcinoma (HCC) sensitivity to chemotherapy. , [ ] .

Techniques: Migration

Journal: Bioengineering

Article Title: Mesenchymal Stem Cell-Mediated Targeted Drug Delivery Systems for Hepatocellular Carcinoma: Current Advances and Future Directions

doi: 10.3390/bioengineering12111206

Figure Lengend Snippet: Immunomodulatory effect of MSCs in the hepatocellular carcinoma microenvironment. This schematic diagram unravels the cytokine-mediated interaction network within the hepatocellular carcinoma microenvironment regulated by MSCs. The upper section (light green) details the macrophage dynamic: pro-inflammatory M1 cells release IL-1β and IL-6, while M2 cells produce IL-10, modulated by TGF-β’s bidirectional action. The lower section (light yellow) reveals how immunoregulatory molecules (NO, HO-1, and PGE-2) from MSCs target liver cancer cells, while T cells (CD8 + , CD4 + , and Th) contribute cytokines, including IL-6, IL-12, and TGF-β.

Article Snippet: Adipose tissue-derived MSCs (AT-MSCs) , Exosome-mediated miR-122 delivery , Sorafenib , Exosomes regulated target gene expression via miR-122, enhancing hepatocellular carcinoma (HCC) sensitivity to chemotherapy. , [ ] .

Techniques:

Journal: Bioengineering

Article Title: Mesenchymal Stem Cell-Mediated Targeted Drug Delivery Systems for Hepatocellular Carcinoma: Current Advances and Future Directions

doi: 10.3390/bioengineering12111206

Figure Lengend Snippet: Mechanism of engineered MSC-mediated targeted drug delivery for hepatocellular carcinoma therapy. This schematic diagram illustrates the mechanism of targeted drug delivery using mesenchymal stem cells (MSCs) for hepatocellular carcinoma (HCC) therapy. The process begins when HCC cells secrete chemoattractant factors (e.g., SDF-1), which bind to corresponding receptors (e.g., CXCR4) on MSCs, initiating a homing response. Subsequently, these MSCs, which can be genetically engineered and loaded with therapeutic agents (TRAIL, miRNAs, etc.), migrate along the concentration gradient to specifically target the tumor site. Finally, upon arrival, the MSCs release their therapeutic payload directly into the tumor microenvironment, showcasing a sophisticated cell-based delivery strategy.

Article Snippet: Adipose tissue-derived MSCs (AT-MSCs) , Exosome-mediated miR-122 delivery , Sorafenib , Exosomes regulated target gene expression via miR-122, enhancing hepatocellular carcinoma (HCC) sensitivity to chemotherapy. , [ ] .

Techniques: Concentration Assay

Journal: Regenerative Therapy

Article Title: Increasing robustness of in vitro assay for immnosuppressive effect of mesenchymal stromal/stem cells: The role of inflammatory cytokine production by peripheral blood mononuclear cells

doi: 10.1016/j.reth.2024.12.016

Figure Lengend Snippet: Effect of different donor-derived human peripheral blood mononuclear cells (PBMCs) on the in vitro immunomodulatory response of bone marrow (BM)- mesenchymal stem cells (MSCs). (a) Schematic of the protocol for lymphocyte proliferation assays with mitogen as an immunomodulatory potency assay for MSCs. (b) Proliferation of 10 donor-derived PBMCs upon phytohemagglutinin (PHA; 0.2, 0.5, and 1.0 μg/mL) stimulation was assessed through BrdU uptake. BrdU uptake was indicated by luminescence signal (counts per second, CPS). Two-way ANOVA showed that PBMCs proliferation was significantly affected by the stimulating PHA concentration, the presence or absence of MSCs, and their interaction (p < 0.0001). All values indicate the mean ± standard deviation of mean of three biological replicates (n = 3) (∗P < 0.05, ∗∗P < 0.01, ∗∗∗∗P < 0.0001, two-way analysis of variance, followed by Bonferroni's multiple comparison test). (c) Effect of PBMCs derived from 10 donors on the in vitro immunomodulatory response of BM-MSCs. The rate of suppression by BM-MSCs on PBMC proliferation stimulated with three PHA concentrations (0.2, 0.5, and 1.0 μg/mL) is shown as the immunosuppression rate; CPS(PBMC + MSC)/CPS(PBMC).

Article Snippet: Immortalized human adipose tissue-derived MSCs (I-MSC; JCRB1555 HAdpc-26-E6-Bmi-1-TERT) were obtained from JCRB Cell Bank (Ibaraki, Osaka, Japan).

Techniques: Derivative Assay, In Vitro, Potency Assay, Concentration Assay, Standard Deviation, Comparison

Journal: Regenerative Therapy

Article Title: Increasing robustness of in vitro assay for immnosuppressive effect of mesenchymal stromal/stem cells: The role of inflammatory cytokine production by peripheral blood mononuclear cells

doi: 10.1016/j.reth.2024.12.016

Figure Lengend Snippet: Figure summarizing the flow of the reversed effects of MSCs depending on the cytokine production concentration of PHA-stimulated PBMCs in the immunomodulatory potency assay. The illustration of MSC in the figure was done using BioRender: scientific image and illustration software.

Article Snippet: Immortalized human adipose tissue-derived MSCs (I-MSC; JCRB1555 HAdpc-26-E6-Bmi-1-TERT) were obtained from JCRB Cell Bank (Ibaraki, Osaka, Japan).

Techniques: Concentration Assay, Potency Assay, Software